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Home » GAT Subject » Agriculture » Aptitude Test - 12
1. Klenow fragment is derived from:
A. DNA Pol-I
B. RNA Pol II
C. DNA Ligase
D. DNA Pol-I
2.The year of launching of Human Genome Project and completion of the rough draft of the sequence was in:
A. 1992 and 2000
B. 1990 and 2000
C. 1989 and 2001
D. 1992 and 2000
3. The main difference between a plasmid and an episome is:
A. Plasmid is circular episome is linear
B. Episome is circular, plasmid is linear
C. Plasmid can be insert into host genome, episomes are not
D. Plasmid is circular episome is linear
4. What is the final product of the RNaseH method?
A. blunt ended dsDNA
B. staggered dsDNA at both ends
C. staggered dsDNA at 3’ end
D. blunt ended dsDNA
5.The loop region is single stranded. It can be cleaved by using which enzyme?
A. Exonuclease
B. S1 nuclease
C. RNaseH
D. Exonuclease
6. What is the second primer in the case of 5’ RACE?
A. Internal primer
B. Oligo-dA sequence
C. Adaptor-oligo-dT primer
D. Internal primer
7. DNA sequencing is done by
A. Maxam Gilbert method
B. Sanger dideoxy method
C. Both a and b
D. Maxam Gilbert method
8. DNA staining is done by
A. Cystal violet
B. Giemsa staining
C. Methylene blue
D. Cystal violet
9. Use of alkaline phosphatase is to
A. remove terminal phosphates from 3’end
B. remove terminal phosphates from 5’end
C. remove terminal phosphate from both end
D. remove terminal phosphates from 3’end
10.Taq polymerase is used in PCR because of its
A. low thermal stability
B. high fidelity
C. high speed
D. low thermal stability
11.Nick translation is done by
A. DNA polymerase I
B. DNA polymerase II
C. DNA ligase
D. DNA polymerase I
12. Introduction of recombinant DNA into bacterial cell by using current is called
A. Transformation
B. Electroporation
C. Transformation
D. Transformation
13. Telomeric sequences are found in
A. HAC
B. BAC
C. YAC
D. HAC
14. The uptake of plasmid DNA into bacterial cell is facilitated in the presence of
A. Calcium Chloride
B. Magnesium Chloride
C. Potassium Chloride
D. Calcium Chloride
15. Young's modulus is defined as the ratio of
A. volumetric stress and volumetric strain
B. lateral stress and lateral strain
C. longitudinal stress and longitudinal strain
D. volumetric stress and volumetric strain
16.Deformation per unit length in the direction of force is known as
A. strain
B. lateral strain
C. linear strain
D. strain
17.The materials having same elastic properties in all directions are called
A. ideal materials
B. uniform materials
C. isotropic materials
D. ideal materials
18. A thin mild steel wire is loaded by adding loads in equal increments till it breaks. The extensions noted with increasing loads will behave as under
A. uniform throughout
B. increase uniformly
C. first increase and then decrease
D. uniform throughout
19. Modulus of rigidity is defined as the ratio of
A. longitudinal stress and longitudinal strain
B. volumetric stress and volumetric strain
C. lateral stress and lateral strain
D. longitudinal stress and longitudinal strain
20.Tensile strength of a material is obtained by dividing the maximum load during the test by the
A. area at the time of fracture
B. original cross-sectional area
C. average of (a) and (b)
D. area at the time of fracture
21.The Young's modulus of a wire is defined as the stress which will increase the length of wire compared to its original length
A. half
B. same amount
C. double
D. half
22.Percentage reduction of area in performing tensile test on cast iron may be of the order of
A. 50%
B. 25%
C. 0%
D. 50%
23. The intensity of stress which causes unit strain is called
A. unit stress
B. bulk modulus
C. modulus of rigidity
D. unit stress
24. During a tensile test on a specimen of 1 cm cross-section, maximum load observed was 8 tonnes and area of cross-section at neck was 0.5 cm2. Ultimate tensile strength of specimen is
A. 4 tonnes/cm2
B. 8 tonnes/cm2
C. 16 tonnes/cm2
D. 4 tonnes/cm2
25.For steel, the ultimate strength in shear as compared to in tension is nearly
A. same
B. half
C. one-third
D. same
26. If a part is constrained to move and heated, it will develop
A. principal stress
B. tensile stress
C. compressive stress
D. principal stress
27.The value of modulus of elasticity for mild steel is of the order of
A. 2.1xl05 kg/cm2
B. 2.1 X 106 kg/cm2
C. 2.1 x 107 kg/cm2
D. 2.1xl05 kg/cm2
28. The value of Poisson's ratio for steel is between
A. 0.01 to 0.1
B. 0.23 to 0.27
C. 0.25 to 0.33
D. 0.01 to 0.1
29.The buckling load for a given material depends on
A. slenderness ratio and area of cross-section
B. Poisson's ratio and modulus of elasticity
C. slenderness ratio and modulus of elasticity
D. slenderness ratio and area of cross-section
30.The total elongation produced in a bar of uniform section hanging vertically downwards due to its own weight is equal to that produced by a weight
A. of same magnitude as that of bar and applied at the lower end
B. half the weight of bar applied at lower end
C. half of the square of weight of bar applied at lower end
D. of same magnitude as that of bar and applied at the lower end
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