Agriculture :: All Aptitude Test ::

1.Is two crossovers occur between same two strands, this will result in?
A. All recombinant strands
B. Three recombinant strands with different loci exchanged
C. Exchange of middle portion between two strands
D. All recombinant strands

2.Double cross over involving strands result in 100% recombinant strands.
A. 1strand
B. 2 strands
C. 3 strands
D. 1strand

3.How many kinds of mutation are found in DNA which includes mutation of only one base?
A. 1
B. 2
C. 3
D. 1

4.What is the overall rate at which new mutations arise spontaneously at any given site on the chromosome per round of replication?
A. ≈ 10-8 – 10-12
B. ≈ 10-7 – 10-9
C. ≈ 10-7 – 10-9
D. ≈ 10-8 – 10-12

5.What is the overall rate at which new mutations arise spontaneously at any given site on the chromosome per round of replication?
A. ≈ 10-8 – 10-12
B. ≈ 10-7 – 10-9
C. ≈ 10-6 – 10-11
D. ≈ 10-8 – 10-12

6.What is the dinucleotide sequence of micro satellites?
A. CA
B. AT
C. CC
D. CA

7.Micro-satellites involves the repeats of the dinucleotide sequence of CA. the CA repeat is found at many widely scattered sites in the genome of humans and other eukaryotes.
A. Replication
B. Transcription
C. Translation
D. Replication

8.How many steps are required to attain mismatch repair?
A. 1
B. 2
C. 3
D. 1

9.The term ‘endonuclease’ refers to cutting the DNA sequence from?
A. only within the polynucleotide chain, not at the ends
B. only within the polynucleotide chain, not at the ends
C. anywhere in the chain
D. only within the polynucleotide chain, not at the ends

10.The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?
A. By methylation of bacterial DNA by restriction enzyme
B. By methylation of foreign DNA by restriction enzyme
C. By phosphorylation of bacterial DNA by restriction enzyme
D. By methylation of bacterial DNA by restriction enzyme

11.Even after replication, how the modified DNA remains protected?
A. It remains protected because of conservative mode of replication
B. It remains protected because of semi-conservative mode of replication
C. The mode of replication has no role to play in the protection
D. It remains protected because of conservative mode of replication

12.How many classes of restriction enzymes are there?
A. 1 Class
B. 2 Classes
C. 3 Classes
D. 1 Class

13.Which of the following is the use of waxing in plant tissues ?
A. Reduces shrinkage losses
B. Reducing water losses due to evaporation
C. Thick leads to anaerobic respiration conditions and thin coat offers little control over moisture losses
D. Reduces shrinkage losses

14.Which of the following is a permitted food additive by the FDA when not in violation with other section of the act ?
A. Aluminium sodium sulphate
B. Vitamin D-2
C. Sodium polyphosphate
D. Aluminium sodium sulphate

15.Which of the following is NOT true about artificial coloring with respect to food ?
A. It is defined as any material which contains a dye or a pigment
B. This dye or pigment can be obtained by extracting a natural dye or pigment from plants or other sources
C. Caramel falls under its definition
D. It is defined as any material which contains a dye or a pigment

16.The process of expression of foreign genes in a plant is called ?
A. Gene expression
B. Transgenesis
C. Genetic transformation
D. Gene expression

17.Name the strategy where two-plasmid system is used for the introduction of the gene ?
A. Binary vector system
B. Co-integration vector strategy
C. Agrobacterium
D. Binary vector system

18.Which of the following is considered as a visual marker ?
A. Antibiotic marker
B. Resistance marker
C. Selectable marker
D. Antibiotic marker

19.Name the marker gene of streptomycin ?
A. HPT
B. SPT
C. PAT
D. HPT

20.Name the first transgenic virus resistant plant ?
A. Rice
B. Cotton
C. Tobacco
D. Rice

21.Which of the following is supplemented with vitamin A in order to improve its nutritional quality ?
A. Cotton
B. Potato
C. Tomato
D. Cotton

22.Name the enzyme which has silenced to delay the ripening process ?
A. Polygalacturonase
B. Glyphosate
C. ADA
D. Polygalacturonase

23.Name the technique which is used to enhance the life of a tomato ?
A. Antisense technology
B. In vitro gene transfer
C. Ex vivo gene transfer
D. Antisense technology

24.What is the final product of the RNaseH method ?
A. Blunt ended dsDNA
B. Staggered dsDNA at both ends
C. Staggered dsDNA at 3’ end
D. Blunt ended dsDNA

25.What would not happen if the RNA strand is completely removed from RNA: DNA hybrid ?
A. There are no chances of the synthesis of the second DNA strand
B. Chance complementarity would take place
C. Hairpin structure would be formed
D. There are no chances of the synthesis of the second DNA strand

26.The loop region is single stranded. It can be cleaved by using which enzyme ?
A. Exonuclease
B. S1 nuclease
C. RNaseH
D. Exonuclease

27.Choose the correct statement with respect to the self priming method of cDNA synthesis ?
A. It is less preferred than RNaseH method
B. A hairpin structure is formed with guarantee
C. The sequence corresponding to the 5’ end is lost
D. It is less preferred than RNaseH method

28.Choose the incorrect statement for the method homopolymer tailing ?
A. The first step is the RNA: DNA hybrid synthesis
B. Terminal transferase is used for the addition of nucleotides on 3’ end
C. Terminal transferase adds only at DNA strands
D. The first step is the RNA: DNA hybrid synthesis

29. Choose the correct statement for RACE ?
A. It stands for Random Amplification of cDNA ends
B. It is for cloning particular cDNA ends
C. It is only of one type, which is 5’ RACE
D. It stands for Random Amplification of cDNA ends

30.The first primer in the case of 3’ RACE is ?
A. Internal sequence
B. Oligo-dT adaptor molecule
C. Oligo-dA adaptor molecule
D. Internal sequence


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