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Hassan
1.12. Which of the following condition is TRUE for Calotropis?
A. a) Epiphyllous
B. b) Gynandrous
C. c) Epipetalous
D. a) Epiphyllous
2.1. Which of the following phylum is known for its asymmetrical body shape?
A. a) Mollusca
B. b) Arthropoda
C. c) Annelida
D. a) Mollusca
3.An enzyme which synthesis, DNA from RNA is called?
A. DNA polymerase
B. RNA polymerase
C. Nucleases
D. DNA polymerase
4.Name the bacterium from which Taq DNA polymerase derived?
A. Thermus aquaticus
B. Salmonella
C. Cyanobacteria
D. Thermus aquaticus
5.Name the enzyme which degrades DNA molecule by breaking its phosphodiester bonds?
A. DNA polymerase
B. Nucleases
C. Ligase
D. DNA polymerase
6.Which of the following is NOT an endonuclease?
A. Mung bean nuclease
B. S1 nuclease
C. Terminal deoxynucleotidal transferase
D. Mung bean nuclease
7.Which of the following endonuclease has been purified from Aspergillus oryzae?
A. S1 nuclease
B. RNase H
C. RNase A
D. S1 nuclease
8.Name the enzyme which degrade dsDNA after recognizing specific nucleotide sequences?
A. RNase H
B. Restriction endonuclease
C. DNA polymerase
D. RNase H
9.Which of the following is NOT the property of restriction enzymes?
A. Recognizes palindromic sequences
B. Two fold axial symmetry
C. Cut down randomly
D. Recognizes palindromic sequences
10.2. Name the phylum which has a single opening for taking food and throwing out faeces?
A. a) Coelenterate
B. b) Porifera
C. c) Annelida
D. a) Coelenterate
11.Which of the following type of restriction enzyme does not require ATP for cleavage?
A. Type I
B. Type II
C. Type III
D. Type I
12.Name the restriction enzyme which recognizes different sequences, but produces same ends?
A. DAM methylase
B. Isoschizomers
C. Neoschizomers
D. DAM methylase
13.Which enzyme join DNA molecules together?
A. Polymerase
B. Nucleases
C. Ligases
D. Polymerase
14.3. Name the organism which has flame-cell in its excretory system?
A. a) Corals
B. b) Ctenophora
C. c) Roundworm
D. a) Corals
15.Circular DNA used for cloning is called?
A. DNA polymerase
B. RNA polymerase
C. Phagosome
D. DNA polymerase
16.4. Which of the following is causative agent of Trichionosis?
A. a) Trichinella spiralis
B. b) Oxytricha trifallax
C. c) Morus nigra
D. a) Trichinella spiralis
17.Which of the following is NOT a cloning vector?
A. Bacterial plasmid
B. Cosmids
C. Bacteriophage
D. Bacterial plasmid
18.Cloning vector has the following properties, except one?
A. Contain genetic marker for selection
B. Ability to replicate
C. Multiple restriction site for entry of DNA
D. Contain genetic marker for selection
19.Which of the following is NOT the characteristics of the plasmid?
A. Replicate independently in a host cell
B. Selectable marker gene
C. Contain single cloning site
D. Replicate independently in a host cell
20.5. Intestinal fluke disease is caused by phylum__________
A. a) Ctenophora
B. b) Platyhelminthe
C. c) Annelida
D. a) Ctenophora
21.Which of the following is TRUE for YAC?
A. Recognizes palindromic sequences
B. Two fold axial symmetry
C. Recognize specific site according to DNA sources
D. Recognizes palindromic sequences
22.Which of the following is NOT an example of Lambda phages?
A. EMBL3
B. M13
C. λgt10
D. EMBL3
23.Name the vector system used for plant transformation?
A. Agrobacterium
B. Cosmids
C. M13 phage
D. Agrobacterium
24.Name the first vector used for a mammalian cell?
A. Lambda phage
B. SV40
C. M13
D. Lambda phage
25.6. Which organism has segmental nephridia for excretion and osmoregulation?
A. a) Platyhelminthes
B. b) Aschelminthes
C. c) Annelida
D. a) Platyhelminthes
26.Which of the following cloning vector is used for insect and having a DNA genome?
A. Cosmid
B. P-element
C. SV40
D. Cosmid
27.The process by which bacterial cell uptake DNA is called?
A. Gene expression
B. Transformation
C. Transcription
D. Gene expression
28.Which of the following chemical is used in chemical transformation method?
A. Cu
B. FeSO4
C. SO2
D. Cu
29.8. Which of the following phylum has a water vascular system?
A. a) Plantae
B. b) Mollusca
C. c) Echinodermata
D. a) Plantae
30.Which of the following method creates pores in the membrane?
A. Gene gun
B. Chemical transformation
C. Electroporation
D. Gene gun
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