Microorganisms - Section 3 Multiple Questions and Answers.




11.The greatest resolution in light microscopy can be obtained with?
A. Longest wavelength of visible light used
B. An objective with minimum numerical aperture
C. Shortest wavelength of visible light used
D. Shortest wavelength of visible light used and an objective with the maximum numerical aperture

12.Oil immersion objective lens has an NA value of?
A. 0.65
B. 0.85
C. 1.33
D. 1

13.In fluorescence microscopy, which of the following performs the function of removing all light except the blue light?
A. Exciter filter
B. Barrier filter
C. Dichroic mirror
D. Mercury arc lamp

14.Total Magnification is obtained by ?
A. Magnifying power of the objective lens
B. Magnifying power of eyepiece
C. Magnifying power of condenser lens
D. Magnifying power of both the objective lens and eyepiece

15.In light microscopy, which of the following is used as fixatives prior to staining technique?
A. Osmic acid
B. Glutaraldehyde
C. Heat
D. Osmic acid, glutaraldehyde, heat

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