Among all the heat shock proteins which one is known as chaperonins?
A.
Hsp70
B.
Hsp32
C.
Hsp60
D.
Hsp30
Answer: Option C
Explanation:
Hsp60 family of chaperones forms a large barrel-shaped structure that acts later when protein is being fully synthesized. Chaperonins bind incorrectly folded, partly folded or unfolded protein but not in their native state.
Which one of the following reagent is also known a Sanger’s reagent?
A.
1-fluoro-2,4-dinitrobenzene (FDNB)
B.
Phenylisothiocyanate
C.
Cyanogen bromide
D.
β-mercaptoethanol
Answer: Option A
Explanation:
FDNB reacts with a free amino group of N-terminal amino acid in alkaline solution to form yellow dinitrophenol. This reaction was first used by Sanger to determine the primary structure of the polypeptide hormone.
Proteolytic enzyme trypsin cleaves protein at which of these specific sites?
A.
Carboxyl side of Alanine, Glycine
B.
Carboxyl side of Lysin or Arginine
C.
Carboxyl side of aromatic amino acid
D.
The amino side of aromatic amino acid
Answer: Option B
Explanation:
The specificity of these enzymes determined by the side chain of amino acid on either side of a peptide bond. This cleavage is necessary to sequence protein which is made up of more than 50 amino acid residues.
Mark the correct statement which depicts the correct statement of protein assay?
A.
To determine the amount of protein
B.
To characterize the function of the protein
C.
To determine the structure of the protein
D.
To determine the sequence of the protein
Answer: Option A
Explanation:
Protein assay is a technique to determine the amount of protein in an unknown solution. It is done by measuring the absorbance at 280nm. Aromatic side chain containing amino acids like tyrosine, tryptophan, and phenylalanine exhibit strong UV light absorbance.
In a living organism, strong interactions between molecules are formed by a certain type of bond. Name that?
A.
Hydrogen bonding
B.
Covalent bond
C.
Electrostatic interactions
D.
Van der Waals forces
Answer: Option B
Explanation:
Covalent bond is considered as a strong bond between molecules whereas rest options are of the non-covalent bond which is regarded as weak interactions. In a covalent bond, electrons are shared by both the atoms which makes it stronger.
